Chaoqin REN, WeiYUAN, Xianzhi DAI, Yuan LIU
Abstract：[Objective]To select the optimal extraction technology of flavonoids components from RADIX MOGHANIAE. [Methods] RP-HPLC method was adopted for the screening. Chromatographic column was Diamonsil C18 (250mm×4.6mm,5μm); mobile phase was an hydrous methanol 0.5% phosphoricacid,0→10min(23:77,V/V) ,11→55min(43:57,V/V) ; flow rate was 1 mL/min; column temperature was 30℃ ; and detection wave length was 240 nm. [Results] The optimal extraction technology of total flavonoids from RADIX MOGHANIAE was as follows: 1:16 solid-liquid ratio,80℃reflux extraction for 2h,and 3 extraction times.[Conclusions]This research provided references for the further research and extraction of RADIX MOGHANIAE.
Keywords:RADIX MOGHANIAE; Extractiontechnology; RP-HPLC