Zhifeng Zhang, Luyang Lu, Yuan Liu
Nat Prod Res.2014,28(15):1171-1175.
Abstract:In the study, the major alkaloids from Fritillariae Hupehensis Bulbs (FHB) have been analysed for the first time by the combined use of the following two methods: the simultaneous quantitation of three alkaloids by using high-performance liquid chromato graphy coupled with evaporative light scattering detector (HPLC-ELSD) and the simultaneous characterisation of seven alkaloids by using HPLC coupled with electro sprayionisation-mass spectrometry analysers detection (HPLC-ESI-MSn). The other fourcon generic species were compared by using the established method. Both correlation coefficients of similarity in chromato grams were calculated for quantitative expression of HPLC profiles. The results revealed that the chromatogram profile combining similarity evaluation could efficiently identifyand distinguish FHB from other different origins of Fritillaria species. The established method was considered to be suitable for checking the genuine origin and quality control of FHB.
Keywords:Fritillariae;Hupehensis Bulbs; congeneric plants;alkaloids; HPLC-ELSD; HPLC-ESI-MSn