Wang X, Zhou G, Xu X, Geng R, Zhou J, Yang Y, Yang Z,Chen Y
Abstract:Recent studies in domestic animals have used RNA-seq to explore the transcriptome of different tissues in alimited number of individuals. In the present study, de novo transcriptome sequencing was used to compare sheep adipose tissue transcriptome profiles between a fat-tailed breed (Kazak sheep; KS) and a short-tailed (Tibetan sheep;TS). The RNA-seq data from these two groups revealed that 646 genes were differentially expressed between the KS and TS groups, including 280 up-regulated and 366 down-regulated genes. We identified genes relevant to fatmetabolism in adipose tissues, including two top genes with the largest foldchange (NELL1 and FMO3). Pathway analysis revealed that the differentially expressed genes between the KS and TS breeds belong to fatty acid metabolism relevant pathways (e.g. fat digestion and absorption, glycine, serine, andthreonine metabolism) and cell junction-related pathways (e.g. cell adhesionmolecules) which contribute to fat deposition. This work highlighted potentialgenes and gene networks that affect fat deposition and meat quality in sheep.