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Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry
2015-09-02 15:41     (点击次数:)

Li shanshan,Wang jiaqi, Wei hongyang, Yang yongxin, Bu dengpan, Zhang leying, Zhou lingyun

Agricultural Sciences in China.2012,11(3):101-105.

Abstract:Protein phosphorylation is an important post-translational modification that regulate smilk protein structure and function.The objective of this study was to analyze the presence of phosphorylated casein.Bovine milk proteins were first separated by SDS polyacrylamide gel electrophoresis.After in gels digestion and extraction,phosphorylated peptides were enriched by titanium dioxide and identified by ultra performance liquid chromatography coupled with nano electrospray ionization tandem mass spectrometry.This method ensured the identification of 20 phosphorylated peptides,including 7 phosphorylated forms of α s1-casein,8 α s2-casein,and 5 β-casein.Eight phosphorylated sites derived from 3 α s1-caseins,3 α s2-caseins,and 2 β-caseins were also identified,and localized on residues Ser61,Ser63 and Ser130 in α s1-casein;Thr145,Ser146 andSer158 in α s2-casein;and Ser50 and Thr56 in β-casein.These findings provide valuable information for investigating casein phosphorylation of the bovinemilk.

Keywords:bovinemilk; casein; phosphorylation; mass spectrometry; titanium dioxide; enrichment

原文链接:http://www.docin.com/p-743310086.html

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