Chunyu Bai, Dianjun Wang, Xiaohua Su, Minghai Zhang,Weijun Guan, Yuehui Ma
Turkish journal of veterinary & animal sciences,2012,36(6):659-667.
Abstract:Using Jining Greygoat ear marginal tissues as experimental materials, we succeeded in establishing a fibroblast cell bank, including 32 samples, by means of a primaryex plant and cryopreservation technique. The results from the biological analysis suggested that the population doubling time of the cell line was approximately48 h. The diploid accounted for 98.35%, the isozyme analysis of the lacticde hydrogenase and malicde hydrogenase disproved cross contamination, and the results of the bacterium, fungi, virus, and mycoplasma detection tests were all negative. The transfection rates of 3 fl uorescent protein genes were high,which supported the opinion that exogenous genes could be eff ectively expressed in the cells. This research has not only preserved the precious germplasm resources of the Jining Grey goat at the somatic cell level, but it has also provided valuable materials for the study of genomics, postgenomics,and somatic cloning.
Keywords:Jining Grey goat; fibroblastcell bank; cryogenic preservation; genetic conservation