PengFei Hu, XiangChen Li, XianWei Chen, WeiJun Guan, YueHui Ma
Life Science Journal, 2012,9(4):2309-2312.
Abstract:Huoyan goose is a famous Chinese local breed for its egg productivity. In this study, a bacterial artificial chromosome library of the Huoyan goose was successfully established using the Hind III site of the vector pBeloBAC11, comprising of 115, 200 clonesarrayed in 543, 384 well microplates , with an average insert size of 102 kb and the content of the library was 11.4 genome equivalents, which yielded atheoretical probability of 99.93% for isolating a particular DNA sequence. BACclones of the library were stable in the bacterial host for at least 100passages.
Keywords:Bacterial artificialchromosome library; Huoyan goose; genomic DNA