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Construction and Characterization of a Bacterial Artificial Chromosome Library from the Huoyan Goose
2015-09-02 11:41     (点击次数:)

 

PengFei Hu, XiangChen Li, XianWei Chen, WeiJun Guan, YueHui Ma

Life Science Journal, 2012,9(4):2309-2312.

Abstract:Huoyan goose is a famous Chinese local breed for its egg productivity. In this study, a bacterial artificial chromosome library of the Huoyan goose was successfully established using the Hind III site of the vector pBeloBAC11, comprising of 115, 200 clonesarrayed in 543, 384 well microplates , with an average insert size of 102 kb and the content of the library was 11.4 genome equivalents, which yielded atheoretical probability of 99.93% for isolating a particular DNA sequence. BACclones of the library were stable in the bacterial host for at least 100passages.

Keywords:Bacterial artificialchromosome library; Huoyan goose; genomic DNA

原文链接:http://www.lifesciencesite.com/lsj/life0904/340_12362life0904_2309_2312.pdf

 

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