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Molecular characterization and phylogenetic analysis of the genome of porcine torovirus
2015-06-11 16:28     (点击次数:)

SunHuan, Lan-Daoliang, Lu Li, Chen-Molin, Wang-ChengSong, Hua-XiuGuo.  

ArchVirol,2013, Oct 12.(Impact factor 2.03, SCI)

Abstract: In this study, we amplified and sequenced the first genome of porcine torovirus(PToV SH1 strain). The genome was found to be 28,301 bp in length, sharing 79% identity with Breda virus. It mainly consists of replicase(20,906 bp) and structural genes: spike (4,722 bp), membrane(702 bp), hemagglutinin-esterase (1,284 bp), and nucleocapsid(492 bp). Sequence alignments and structure prediction suggest genetic differences among toroviruses, mainly in NSP1 (papain-like cysteine proteinase domain). Rooted phylogenetic trees were constructed based on the 3C-like proteinase and RNA-dependent RNA polymerase genes. PToV, Berne virus and Bredavirus were clustered together, forming a separate branch from white bream virus that was distant from that of the coronaviruses.

Keywords:Porcine torovirus, Genome, China, Comparative analysis

原文链接:http://link.springer.com/article/10.1007/s00705-013-1861-x

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