Xiong-XianRong, Lan-DaoLiang, Li Jian*, Wang Yong, Zhong-JinCheng.
Zygote, 2013,12:1-8. (Impact factor 1.5, SCI)
Interspeciessomatic cell nuclear transfer (iSCNT), a powerful tool in basic scientificresearch, has been used widely to increase and preserve the population ofendangered species. Yak (Bos grunniens) is one of these species. Development toterm of interspecies cloned yak embryos has not been achieved, possibly due toabnormal epigenetic reprogramming. Previous studies have demonstrated thattreatment of intraspecies cloned embryos with (NaBu) significantly improvesnuclear-cytoplasmic reprogramming and viability in vitro. Therefore, in thisstudy, we evaluated the effect of optimal NaBu concentration and exposure timeon preimplantation development of yak iSCNT embryos and on the expression patternsof developmentally important genes. The results showed that 8-cell rate,blastocyst formation rate and total cell number increased significantlycompared with their untreated counterparts when yak iSCNT embryos were treatedwith 5 nM NaBu for 12 h after activation, but that the 2-cell stage embryo ratewas not significantly different. The treatment of NaBu also increasedsignificantly the expression levels of Oct-4 and decreased the expressionlevels of HDAC-2, Dnmt-1 and IGF-1; the expression patterns of these genes weremore similar to that of their bovine-yak in vitro fertilization (BY-IVF)counterparts. The results described above indicated that NaBu treatmentimproved developmental competence in vitro and 'corrected' the gene expressionpatterns of yak iSCNT embryos.
Keywords:Development;Expression; Sodium butyrate; Yak; iSCNT