青藏高原生态畜牧业协同创新中心
设为首页  |  加入收藏
 
 首 页  中心概况  组织机构  平台建设  人才培养  科学研究  创新团队  人才招聘  资料下载  中心信箱 
当前位置: 首 页>>科学研究>>成果展示>>论文专著>>2013年度>>正文
Zebularine and scriptaid significantly improve epigenetic reprogramming of yak fibroblasts and cloning efficiency.
2015-06-11 16:35     (点击次数:)

Xiong-XianRong, Lan-DaoLiang, Li Jian*, Zhong-JinCheng, Zi XiongDong, MaLi, Wang Yong.

CellReprogram,2013, 15(4): 293-300. (Impact factor 2.744, SCI)

Abstract: Abnormal epigenetic reprogramming of the donor nucleus after somatic cell nucleartransfer (SCNT) is thought to be the main cause of low cloning efficiency.Following SCNT, the donor nucleus of ten fails to express early embryonic genesand establish a normal embryonic pattern of chromatin modification. Therefore,in this study, we have attempted to improve epigenetic reprogramming of the donor nucleus and cloned embryos with Zebularine and Scriptaid. Yak fibroblasts were treated with 20μM Zebularine alone or 20μMZebularine plus 0.5μMScriptaid for 24 h, whereas yak cloned embryos were treated exclusively with 0.5μM Scriptaid for12 h. There was no effect on cellular viability and proliferation after drug treatment. The treatment of fibroblasts with Zebularine or Zebularine plusScriptaid increased histone acetylation of histone 3 lysine 9 (H3K9), but decreased the level of DNA methylation of Oct-4 and Sox-2 promoter regions.When donor cells were used after Zebularine plus Scriptaid treatment toreconstruct cloned embryos and then treated with Scriptaid, the developmental competence and cryosurvival of embryos were improved significantly. In addition, the relative expression of Oct-4 and significantly decreased when fibroblasts and cloned embryos were treated with Zebularine or Scriptaid. This work provides functional evidence that treatment with Zebularine and Scriptaid modifies the epigenetic status of yak fibroblasts, subsequently enhancing invitro developmental potential and the quality of yak cloned embryos.

原文链接http://www.ncbi.nlm.nih.gov/pubmed/23790013

上一条:Oocyte extract improves epigenetic reprogramming of yak fibroblast cells and cloned embryo development
下一条:Sodium butyrate improves the cloned yak embryo viability and corrects gene expression patterns.
关闭窗口
武侯校区地址:四川省成都市一环路南四段16号(610041)航空港校区地址:双流县航空港开发区大件路文星段168号(610225)
西南民族大学版权所有   蜀ICP备11019012号