Xianrong Xiong, Jian Li*, Mei Fu, et al
Abstract: The objective was to investigate the effects of bovine oocyte extract (BOE) on epigenetic reprogramming of yak fibroblast cells, based on their cell cyclestatus, histone acetylation, DNA methylation, gene expression, and cloned blastocyst formation. Permeabilization of yak fibroblasts after treatment with10 or 50 μL of BOE (treated-S and treated-L groups, respectively) for 24 hours increased (P < 0.05) the cell population at the G0/G1 phase(85.2 ± 2.3% and 89.6 ± 1.5%, respectively) compared with controls (75.4 ±1.1%). Acetylation at lysine 9 of histone H3 was also higher (26.1 ± 1.4 and33.5 ± 2.1) than in the control group (15.3 ± 1.6; P < 0.05). Moreover, BOE reduced methylation of the promoter regions of Oct-4 and Nanog (76.4%and 72.2%; and 35.6% and 30.0%, respectively) compared with the control group(92.1% and 47.8%; P < 0.05). In addition, the relative expression levels of HDAC-1, HADC-2, Dnmt-1, and Dnmt-3a were down regulated (P < 0.05) after yak fibroblasts were treated with BOE.Further more, when yak fibroblasts were used for interspecies somatic cellnuclear transfer after BOE treatment, 8-cell and blastocyst formation rates significantly exceeded those of the control. In conclusion, BOE induced epigenetic reprogramming of yak fibroblasts, making them suitable donors for yak interspecies somatic cell nuclear transfer.
Keywords:Extract; Gene expression; Reprogramming; Yak