青藏高原生态畜牧业协同创新中心
设为首页  |  加入收藏
 
 首 页  中心概况  组织机构  平台建设  人才培养  科学研究  创新团队  人才招聘  资料下载  中心信箱 
当前位置: 首 页>>科学研究>>成果展示>>论文专著>>2013年度>>正文
Lipidomic Fingerprint of Almonds (Prunus dulcis L. cv Nonpareil) Using TiO Nanoparticle Based Matrix Solid-Phase Dispersion and MALDI-TOF/MS and Its Potential in Geographical Origin Verification.
2015-06-11 17:32     (点击次数:)

QingShen, Wei Dong, Mei Yang, Li-n-qiu Li, Hon-yeung Cheung*, Zhi-feng Zhang*.

JAgric Food Chem. 2013. 61, 7739−7748. (Impactfactor 2.906, SCI)

Abstract:Amatrix solid-phase dispersion (MSPD) procedure with titanium dioxide (TiO2)nanoparticles (NP) as sorbent was developed for the selective extraction of phospholipids from almond samples, and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF/MS) was employed for analysis.A remarkable increase in the signals of phospholipid accompanied by a decrease in those of triacyl glycerols and diacylglycerols was observed in the relevant mass spectra. The proposed method was applied to five batches of almonds originating from four geographical areas, whereas principal component analysis(PCA) was utilized to normalize the relative amounts of the identified phospholipid species. The results indicated that the lipidomic fingerprint of almonds was successfully established by the negative ion mode spectrum, and theratio of m/z 833.6 to 835.6 as well as m/z 821.6 could be introduced as potential markers for the differentiation of the tested almonds with different geographical origins. The whole method is of great promise for selective separation of phospholipids from nonphosphoipids, especially the glycerides, and superior in fast screening and characterization of phospholipids in almond samples.

Keywords:lipidomicfingerprint;TiO2;matrix;solid-phasedispersion;principalcomponent analysis;MALDI-TOF/MS;almond

原文链接:http://pubs.acs.org/doi/abs/10.1021/jf4016448

上一条:响应面法优化加拿大原产地西洋参皂苷的提取工艺研究
下一条:Phylogenetic lineages of Monopterus albus (Synbranchiformes: Synbranchidae) in China inferred from mitochondrial control region.
关闭窗口
武侯校区地址:四川省成都市一环路南四段16号(610041)航空港校区地址:双流县航空港开发区大件路文星段168号(610225)
西南民族大学版权所有   蜀ICP备11019012号