QingShen, Wei Dong, Mei Yang, Li-n-qiu Li, Hon-yeung Cheung*, Zhi-feng Zhang*.
JAgric Food Chem. 2013. 61, 7739−7748. (Impactfactor 2.906, SCI)
Abstract:Amatrix solid-phase dispersion (MSPD) procedure with titanium dioxide (TiO2)nanoparticles (NP) as sorbent was developed for the selective extraction of phospholipids from almond samples, and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF/MS) was employed for analysis.A remarkable increase in the signals of phospholipid accompanied by a decrease in those of triacyl glycerols and diacylglycerols was observed in the relevant mass spectra. The proposed method was applied to five batches of almonds originating from four geographical areas, whereas principal component analysis(PCA) was utilized to normalize the relative amounts of the identified phospholipid species. The results indicated that the lipidomic fingerprint of almonds was successfully established by the negative ion mode spectrum, and theratio of m/z 833.6 to 835.6 as well as m/z 821.6 could be introduced as potential markers for the differentiation of the tested almonds with different geographical origins. The whole method is of great promise for selective separation of phospholipids from nonphosphoipids, especially the glycerides, and superior in fast screening and characterization of phospholipids in almond samples.