Li Ying, Xia Qing, Liu Yuan, Fuchao Mei
Abstract: Objective:To measure the content of oleanolic acid in Acanthopanax giraldii Harms from different producing areas and different collecting time,A. leucorrhizus var.fulvescens Harms and A. setchuenensis Harms ex Diels from different medicinal parts. Method The HPLC-ELSD method for determination of oleanolic acid was performed on the Kromasil C18 column ( 4.6 mm × 250 mm,5 μm) with the mixture of acetonitrile,water,glacial acetic acid and triethylamine ( 85∶15∶0. 03∶0.05) as the mobile phase at a flow rate of 0.8 mL/min. The column temperature was set at 30 ℃,the ELSD drift tube temperature was set at 78 ℃,and the flow rate of atomization gas high-purity nitrogen was set at 2. 0 L/min. Result The linear range of oleanolic acid was 2.96-118. 50 μg/mL,the regression equation was lgA =1. 2473lgC +3.0069 ( r =0.9991,n =6) ,the average recovery was97.52%,and the RSD was 1.75%. These experimental materials,arranged indescending order of oleanolic acid content,were as follows: A. giraldii Harmsstem bark ( 375. 00 μg/g) > A. leucorrhizus var. fulvescens Harms stem bark( 216.26 μg/g) > A. giraldii Harms root ( 215.63 μg/g) > A. giraldii Harms root bark ( 133.78 μg/g) > A. leucorrhizus var. fulvescens Harms leaf( 35. 53 μg/g) > A. setchuenensis Harms ex Diels stem bark ( 35. 46 μg/g) .No oleanolic acid was found in A. giraldii Harms peeled stem,A. giraldii Harmsleaf and A. setchuenensis Harms ex Diels leaf. The content of oleanolic acid was relatively higher in A. giraldii Harms stem bark collected from March toJuly. As with A. giraldii Harms stem bark,the content of oleanolic acid was the highest in that collected from Majia Ditch,Hongyuan County,AbaPrefecture,Sichuan Province. Conclusion June and July are regarded as the optimal time for harvesting A. giraldii Harms stem bark when they are used as medicine in Sichuan Tibetan and Qiang folk. Majia Ditch,Hongyuan County, AbaPrefecture,Sichuan Province is considered the genuine producing area of A.giraldii Harms,by which the medicinal material containing the highest content of oleanolic acid is produced. It is suggested that A. leucorrhizus var.fulvescens Harms stem bark could serve as the new resource plant for oleanolic acid. It is necessary to carry out in-depth studies about the effects of harvesting time and altitude on the content of oleanolic acid.
Keywords:Acanthopanax giraldii Harms; A.leucorrhizus var. fulvescens Harms; A.setchuenensis Harms ex Diels; Oleanolicacid; HPLC-ELSD