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Application of Morphoanatomy and Microscopy in Authentication of Three Species of Traditional Chinese Herbs of Moghania
2015-06-12 09:10     (点击次数:)

Yan-fei Huang, Chao-qin Ren,Wei Yuan,Yuan Liu*,Zhi-feng Zhang, Lu-yangLu

Pharmacognosy Journal. 2013, 5 : 30-40

Abstract:Background :Moghaniaphilippinensis (Merr. et Rolfe) Li, M. macrophylla (Willd.) O. Kuntze and M.ferruginea (Wall. ex Benth.) Li. (Leguminosae) has not the national standardsnow, and they are just only recorded in appendix of“Pharmacopoeia of the People's Republic of China”(2010 and 2005edition). But their roots are the crude herbs recourses of the primaryingredient in the famous prescription of“Female Moghania Tablet”for curing colpitis in China. Moreover M.philippinensis was also recorded by the appendix“Pharmacopoeia of the People's Republic of China”(1977 edition).M. strobilifera (Linn.) Ait, M. glutiaosa (Prain) Y.T.Wei, and M. LatifoliaBenth are still medical used in some local region of Guangxi and Yunnan Province of China. So their crude herbs recourses are confused in Chinese Crude Herbs Market. Objective: Todistinguish three species of Moghania and ensure their safety and efficacy. Materials and methods :Threespecies of M. macrophylla (Willd.) O.Kuntze, M. Latifolia Benth and M.glutiaosa (Prain) Y.T.Wei from Guangxi, Yunnan and Sichuan Province of China(SWUN) ( Table 1). Fresh materials were fixed in FAA. Samples were passed through the traditiona lethanol and dimethylbenzene series, embedded in paraffin, and then sliced with a microtome and stained with safranine-fast green and finally mounted. The leaflower epidermal cells are obtained by the practical peeling technique and clearing method with nail polish blot. The materials were dissociated by nitricacid chromate method. The samples of herbs were powdered. The average number of palisade cell under the epidermal cells is called Palisade Ratio (PR). Since palisade ratio is consistent and different from plant toplant, this parameter can be used in authentication. Stomatal Index (SI) is abasilic parameter in authentication, which can be obtained from the followingformula: Stomatal Index = (number of stomata/every millimeter-square)×100/(number of stomata/every millimeter-square + number of epidermal cell/everymillimeter-square). Mesophylltissue is divided up by slendest vein, which is called vein islet. The numbersof vein islet in every epidermal cell is called Vein Islet Numbers (VIN), whichalso can be used as a reliable parameter in authentication since it isconsistent in a species of plant. The results were studied using light microscope according to the usual microscopic techniques. Results :The three species of Moghania were studied to compare the distinguishing morphoanatomic details of root, stem, leaf and petiole, dissociation and powders. The microscopic features were systematically described and illustrated. And detailed key authentication parameters based on these anatomic characteristics were presented. A key was constructed to the three species for convenient classification. Microscopy can be unambiguously used to authenticate and distinguish three species of Moghania.

Keywords:Moghania;Microscopy authentication; Morphoanatomy; Traditional Chinese herbs

原文链接:http://www.sciencedirect.com/science/article/pii/S0975357512000041

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